FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_VvMYB13_PN94-B_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_VvMYB13_PN94-B_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21345433
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG22700.019.42701748
TGCCGTC20500.017.58509850
ATGCCGT24650.016.47033949
CGTATGC29950.014.84117746
GCCGTCT18500.014.378159551
CCGCTCA48750.013.13829333
CGCTCAT47950.013.06552634
ACCGCTC49300.012.70774832
CACCGCT50150.012.42257131
TATCTCG48550.011.02969741
TCACCGC56550.011.01668430
CTCGTAT41900.010.85902344
TCTCGTA46700.010.41740143
TCGTATG44950.010.200067545
GTCACCG59600.010.15931329
ACCAACC390800.09.9154991
GGCGCGA12100.09.83458469
ATCTCGT59750.09.31366642
ACCTAAC202800.09.1135471
GTATGCC52350.08.95879547
Produced by FastQC (version 0.11.5)