FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_VvMYB13_PN94-B_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_VvMYB13_PN94-B_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36980166
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG51300.024.08336348
TGCCGTC44700.022.31504450
ATGCCGT52650.021.20570449
CGTATGC60350.020.70383546
GCCGTCT34700.020.47514351
CGCTCAT110550.015.63972834
TATCTCG95950.015.61204541
TCGTATG91600.015.09248945
CCGCTCA114750.015.06729333
CTCGTAT92550.014.89977244
ACCGCTC114950.014.79749832
TCTCGTA98350.013.985543
CACCGCT122250.013.82799631
ATCTCGT114100.013.0365842
GTATGCC102050.012.92969247
TCACCGC133750.012.58674630
GTCACCG144750.011.65443529
CCGTCTT66850.011.046926552
CCGTCGT28700.010.97541652
GCCGTCG31850.010.43937751
Produced by FastQC (version 0.11.5)