FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_VvMYB135_PN94-B_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_VvMYB135_PN94-B_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42449854
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC38000.013.447165550
TATGCCG47550.013.10176948
CGTATGC55250.011.52921146
ATGCCGT51450.011.49642549
GCCGTCT32550.011.18262651
TATCTCG93350.08.51083141
CCGCTCA101500.08.20678233
CGCTCAT99950.08.05390534
TCTCGTA96900.07.98230743
ACCGCTC101100.07.92768432
CTCGTAT91000.07.80754644
TCGTATG87750.07.737756345
CACCGCT108400.07.36151231
ATCTCGT115450.07.093856342
TCACCGC117350.06.889550730
GTATGCC107100.06.43779647
GTCACCG128100.06.33870929
CCGTCTT62350.06.286994552
CCGTCGT26900.06.245258352
GCCGTCG35000.05.099922751
Produced by FastQC (version 0.11.5)