FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_VvMYB135_PN94-B_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_VvMYB135_PN94-B_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19089533
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20250.010.71590248
TGCCGTC17250.010.14478950
GCCGTCT14600.09.82863351
CGTATGC24650.09.51305546
ATGCCGT23250.09.1826749
CGCTCAT41750.07.20948234
ACCGCTC41800.07.20085832
CCGCTCA43350.06.943388533
CACCGCT44850.06.789204631
TATCTCG40700.06.70752541
CTCGTAT39100.06.623950544
TCGTATG40000.06.212398545
TCTCGTA41900.06.18129943
TCACCGC48950.05.934545530
GTCACCG56550.05.44643329
ATCTCGT52550.05.328185642
Produced by FastQC (version 0.11.5)