FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_VvHY5_PN94amp-B_r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_VvHY5_PN94amp-B_r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137307470
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG460300.022.27858539
CATCGGG600750.021.74247438
GCGGGGT186500.021.46886649
GCATCGG675300.019.886437
GCGCATC1009650.019.78677435
CGCATCG886200.019.32039536
TGGCGGG208650.019.25683647
TGCGCAT1248100.017.23474734
TCGGGGT292150.016.34065440
CGGGGTT270400.016.23124341
CGGGGGT226150.016.07977341
CGGGGTG284150.015.38426250
ATGCGCA1492900.015.16824433
CATCGCG221850.015.1293538
TGCGGGG162900.014.438086548
TTGCGGG195650.013.57761247
AATGCGC1708750.013.46520332
GGCGGGG305700.013.16630348
TCGGGGG321450.012.76071840
TAATGCG1924050.011.98937531
Produced by FastQC (version 0.11.5)