FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_VvHY5_CS8amp-B_r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_VvHY5_CS8amp-B_r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117929759
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGTT2673260.22668239320322872TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGGT2108650.17880558884208353TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1540180.13060147099935987No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG1202050.038.9869942
CATCGGG1577300.038.1748241
GCATCGG1669800.037.05362340
GCGCATC2438450.036.9063738
TGCGCAT2651050.036.15405737
GCGGGGT570950.036.13662752
CGGGGTG593150.036.05271553
CGCATCG2204550.035.48135439
ATGCGCA2876350.034.94781536
TGCGGGG360450.034.21786551
AATGCGC2988950.034.13013535
CGGGGGT498400.033.00509344
TCGGGGT644750.032.63538443
TAATGCG3135300.032.5916934
TCGGGGG579700.031.54598643
CGGGGTT637650.031.03377344
CTAATGC3497650.029.36933933
CATCGCG515750.028.6985741
TGGCGGG684650.027.49249850
ACTAATG3881450.026.6284932
Produced by FastQC (version 0.11.5)