FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_TGA5_Colamp-B_ryq269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_TGA5_Colamp-B_ryq269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5205035
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCATC15050.035.813659
GTGGCCA18950.030.8440657
GCCATCT19850.028.03502710
TGGCCAT22500.025.3553418
CCGTCCA13500.019.1850227
GGTGGCC9400.017.1289946
CGTCCAT15400.016.1362278
GTCCATC15100.015.0660979
CCATCTG18700.014.97313411
CGTGGCC15150.014.7866336
GCGATAG12550.014.22298651
GTATGCC13350.013.63284363
GCCGTCT11800.013.34734367
AGTGGCC8450.012.8412376
CACAGCG14850.012.72716447
CTGATCG15450.012.00648615
CCATCTA17550.011.9657111
TGATCGG16650.011.56157516
GCCGTCC8550.011.4628836
AGCGATA16350.011.13140450
Produced by FastQC (version 0.11.5)