FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_SPL16_Colamp-B_r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_SPL16_Colamp-B_r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8321063
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC25800.045.30956638
AATGCGC30250.044.19785335
TGCGCAT28900.043.11370537
ATGCGCA30550.042.8473136
CGCATCG25750.037.921939
ATCGGGG16000.037.84309842
GCATCGG20500.037.56055540
TAATGCG36300.037.02415534
CAGTCAC39800.034.55970427
CGGGGTG8750.033.1994353
ACGTCTG41650.033.02543315
TCCAGTC41950.032.78866625
CCAGTCA42300.032.3516926
CACGTCT45050.030.76602414
GTCACTA44950.030.52227629
CTCCAGT45400.030.29701824
CGTCTGA46150.030.10854516
CATCGGG24700.030.0401541
GCACACG47550.028.92729211
CTAATGC47450.028.39782133
Produced by FastQC (version 0.11.5)