FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_PpBSD_Pp-B_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_PpBSD_Pp-B_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18318976
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT303810.1658444227450268TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG60450.040.9918548
TGCCGTC49450.039.3522150
CGTATGC64500.039.01483546
ATGCCGT59750.038.89473349
GCCGTCT36700.038.1464951
TCGTATG74200.034.38620845
GTATGCC74250.034.08022747
CATCTCG74550.031.73654741
GTCACGA101600.031.69271729
AGTCACG101800.031.63028128
CTCGTAT79400.030.19469844
CACGAGA110150.029.42317231
CGAGATT111250.028.66034133
TCACGAG117400.027.45716730
CAGTCAC119650.027.17485427
ACGAGAT120050.026.82184432
TCTCGTA85700.026.54563943
CCGTCTT56150.025.05745352
ATCTCGT95400.025.05722442
AGATTCC139450.023.14064235
Produced by FastQC (version 0.11.5)