FastQCFastQC Report
Wed 2 Dec 2020
HTW7MBGXG_n01_PpBSD_Pp-B_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW7MBGXG_n01_PpBSD_Pp-B_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10500244
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT174300.1659961425658299TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG34900.044.72734548
TGCCGTC29600.041.9759350
CGTATGC39100.040.4599546
ATGCCGT36150.040.2762249
GCCGTCT20950.039.92799851
GTATGCC42700.037.37667547
TCGTATG42850.037.24583445
CTCGTAT45000.034.610644
CATCTCG44900.034.37588541
AGTCACG60200.033.83703228
GTCACGA60550.033.4681929
CACGAGA64650.031.5619431
TCTCGTA47850.031.08626743
CGAGATT65100.031.07495533
TCACGAG69050.029.75378830
ACGAGAT70750.028.840732
CAGTCAC70600.028.803127
ATCTCGT54950.028.2161442
AGATTCC80800.024.77697835
CCAGTCA84350.024.39844126
Produced by FastQC (version 0.11.5)