FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35102780
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5829811.6607829921162938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC23400.09.1521411
TGCGCGA23850.08.5971359
CCGGGAT43100.08.391941
CGGTTCG12450.08.23470612-13
CGGGAAT23200.08.0002341
CCGATCT29900.07.96068952
GATCGGT23400.07.7394295
CCGGTAT21400.07.56120731
GTGTCGA16058.185452E-117.421674
CGGAAAT30800.07.10776661
ACACGTG45500.06.9114514
CGATCCG16800.06.902221778-79
CTGGAAT109750.06.76466231
GAATCCG44600.06.7304234
GGGATCG38900.06.4888453
GTGCGCG37700.06.450088
CCCGTAT15651.4985199E-76.38603071
GGAGAAT110650.06.32254551
TCGAATC19104.2018655E-96.23101142
GGGGAAT71100.06.22500041
Produced by FastQC (version 0.11.5)