FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n02_ALm16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n02_ALm16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23056019
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA39100.013.13042459
GTTACGA19500.011.4503234
TAACACG74450.08.9298162
CGGGAAT46500.08.8846981
ACGTGCG89500.08.8677986
CGATCCG29800.08.84157578-79
CTACCGT25850.08.8264888
CCGGTAT45250.08.8152991
TACGACG29800.08.7736079
TCGACCG32600.08.60207926-27
AATCCGA38150.08.5943065
GTGTCGA28950.08.5331484
GTGCGCG65850.08.5179188
TTACGAC23550.08.4745115
CCGGGAT94200.08.3682281
AACACGT82850.08.3122893
ATCCGAC28450.08.18532856
TGTTACG24400.08.1753173
CGCGGAT25650.08.1459481
CGACCTA10556.2951585E-88.1054024
Produced by FastQC (version 0.11.5)