FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n02_ALm15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n02_ALm15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26574093
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA47600.014.0806919
GTTACGA23700.011.2248124
CGATCCG37150.09.84031278-79
TCGACCG39050.09.73709226-27
GTGCGCG79750.09.655328
TAACACG86100.09.6518182
TTACGAC30200.09.4401795
TGTTACG28250.09.4148483
ACGTGCG109550.09.3266886
GTGTCGA33950.09.0952164
TACGACT31350.08.7920846
CGGGGTT95500.08.7019271
AACACGT100850.08.665343
GTTCGAC45550.08.65928624-25
CGCGAGT47950.08.07988210-11
CAGACGT80000.08.0740733
CTACCGT30900.07.9988448
CTCGGAT89150.07.9900721
TCGGATT48200.07.783132
GATCGGA321400.07.695653494-95
Produced by FastQC (version 0.11.5)