FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n02_ALm14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n02_ALm14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34241171
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA57700.013.1820779
ACGTGCG125000.09.542986
TAACACG101500.09.2164782
GTGTCGA39300.09.1874
CGATCCG44450.08.971415578-79
GTGCGCG93200.08.9253198
TCGACCG45600.08.85971626-27
AACACGT114100.08.5332213
GTTACGA30300.08.4664984
CGCGTAT7301.8508084E-58.4566931
CCGGGAT129250.08.3401791
TACGACG44950.08.2490639
CGGGAAT69900.08.2203111
CAGACGT95800.08.0810333
CGGGGTT121400.08.0580241
GCGTCGT257350.07.848878494-95
TGTCGAG50500.07.80964855
GTTCGAC53200.07.6822124-25
CGTTCGA44750.07.647037
TGTTACG33700.07.61042073
Produced by FastQC (version 0.11.5)