FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n02_ALm13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n02_ALm13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31221240
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA61050.011.4456679
GTTACGA29500.010.30649954
ATCCGAC40050.09.7299546
GTGTCGA39400.09.6459944
TTACGAC35150.09.0570245
CGATCCG42300.08.92265178-79
CGGGGTT112800.08.8831971
TGTTACG36200.08.7905583
AATCCGA51000.08.7577745
CGCGATT13107.2759576E-128.7003331
TACGACT37150.08.6986046
GTCTAAC68350.08.6880994
TAACACG105950.08.6054712
TCGACCG45100.08.5363826-27
AACACGT118550.08.5335213
TGTCGAG47100.08.4741175
ACGTGCG127300.08.3995026
GTGCGCG98300.08.2201128
CCGGGAT130000.08.1462441
CAGACGT93400.08.0853813
Produced by FastQC (version 0.11.5)