FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n02_ALm12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n02_ALm12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30409862
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA56250.012.5919889
CTACCGT38200.011.4479918
GTGTCGA38350.011.02526954
TCGACCG39900.010.06591426-27
ACGTGCG112150.010.0433046
TGTCGAG46700.09.7680265
TAACACG98450.09.4057342
GTTACGA27050.09.308364
CGATCCG41100.09.29842278-79
TACCGTA39550.08.89438159
CCGGGAT119200.08.8839911
AACACGT110800.08.7018193
CCGGTAT61300.08.5988831
GTTCGAC48400.08.54254224-25
GTGCGCG90550.08.5041598
CGGGGTT106200.08.4064531
TTACGAC30450.08.1146275
GCGTCGT181850.07.922840694-95
CGCGGAT34600.07.82308861
TGTTACG29800.07.8096873
Produced by FastQC (version 0.11.5)