FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n02_ALm11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n02_ALm11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38301010
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA72900.013.4330089
GTTACGA34800.011.7403614
TTACGAC39900.010.956235
TGTTACG38100.010.8457193
TACGACT43650.010.4521286
CTACCGT41250.09.1035228
GTGCGCG127050.09.091558
ACGTGCG173450.09.0692516
TAACACG144850.09.0482832
CGATCCG53050.08.904242578-79
ATCCGAC52100.08.66576
TAGCCGA59350.08.57031259
AACACGT161900.08.4490853
CCGGTAT87900.08.4276011
GTCTAAC76300.07.9698054
TCGACCG59700.07.842127326-27
ACACGTG181150.07.7365254
CGGGAAT78300.07.6414711
AATCCGA62150.07.5690315
ACGACTT66650.07.55903967
Produced by FastQC (version 0.11.5)