FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n02_ALm10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n02_ALm10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19566181
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA36650.011.8028159
GTGTCGA26300.011.5604764
TACGACT22500.011.1943336
GTTACGA19750.011.0647674
TTACGAC21300.010.4845765
TGTTACG23300.010.1918593
CCGGGAT80150.09.0647341
ACGTGCG77600.08.69623856
TAACACG64900.08.63373852
TGTCGAG32850.08.5339345
GATCGGT45800.08.2995995
GTGCGCG60250.08.126038
TGTGTCG39000.08.03745653
AACACGT74800.07.8733483
CGCGGAT23550.07.86395171
ACGACTT36650.07.78090147
CTACCGT23450.07.7026298
ACACGTG82650.07.7021764
GTCGAGG48550.07.63502076
CGAGAAT38050.07.6127641
Produced by FastQC (version 0.11.5)