FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n02_ALm09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n02_ALm09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25214022
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA49100.012.0046019
GTGTCGA34850.010.9055634
CCGGTAT49500.09.6895561
ACGTGCG100050.09.1673596
TGTCGAG39550.09.1305145
TCGACCG35950.09.05626626-27
CGGTATT55150.09.0407732
CGATCCG34000.08.86681978-79
GTTCGAC38400.08.84875924-25
TAACACG85100.08.7605612
GTTACGA21600.08.5777184
ATCCGAC32950.08.5094346
GTGCGCG76100.08.3070848
CGGGGTT91000.08.1930561
AACACGT95100.08.0407383
TGTTACG24350.07.99715383
AATCCGA43650.07.9463335
TTACGAC25350.07.87226065
CCGGGAT101350.07.8717841
CGCCAAT37250.07.64914041
Produced by FastQC (version 0.11.5)