FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n02_ALm08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n02_ALm08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24563836
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA43150.011.6770599
GTTACGA22650.010.4869054
GTGTCGA30800.010.1797894
CGATCCG31400.09.67671378-79
TTACGAC25100.09.0863255
TACGACT27800.09.0598296
TGTTACG26000.08.5857813
ACGTGCG91550.08.4090246
GCGTCGT140800.08.21017194-95
TAACACG75900.08.1336782
TGTCGAG36450.08.0819275
TCGACCG35900.08.009726-27
CTACCGT27450.07.9651638
GTTCGAC40000.07.900789324-25
ATCGGAA334750.07.84260694-95
GATCGGA337050.07.77500494-95
CCGGGAT101250.07.73874951
GTCTAAC47200.07.649224
GTGCGCG74100.07.56907848
GGGGAAT173800.07.4592431
Produced by FastQC (version 0.11.5)