FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n02_ALm07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n02_ALm07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31689468
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA61000.011.9226539
GTGTCGA41950.011.2113174
TGTCGAG49200.010.1403855
TAACACG103750.09.4287072
CGATCCG40550.09.42487578-79
ACGTGCG128850.09.0360756
CTACCGT38100.08.982388
AACACGT115550.08.8786453
TCGACCG44850.08.68976126-27
ATCCGAC44850.08.1588026
GTCTAAC63500.08.1546664
CGGGAAT62500.08.1300761
GTGCGCG99700.08.0570388
CGGGGTT111200.08.0286761
GTTCGAC50500.07.810682324-25
CCGGGAT136450.07.72627741
GCGTCGT204850.07.68235194-95
GATCGGA494000.07.63027494-95
TACGACG43050.07.6188139
ACACGTG134900.07.50103244
Produced by FastQC (version 0.11.5)