FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n02_ALm06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n02_ALm06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32318132
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA59450.012.2336989
GTTACGA30950.011.5120064
TTACGAC32800.010.7197585
TGTTACG35150.010.1341083
TCGACCG46500.09.96587226-27
CGATCCG46100.09.68026578-79
TACGACT38800.09.6761336
TAACACG111400.09.1223812
ACGTGCG135200.08.9281916
CCGGTAT68400.08.8173221
GTGTCGA40450.08.6908774
CGGGGTT121150.08.5843961
CCGGGAT134700.08.49646951
CGGTATT72000.08.3762742
GTTCGAC56750.08.33220624-25
AACACGT126600.08.2535073
CGTTCGA47700.08.1705077
GTGCGCG96200.08.152658
GCGTCGT191200.08.10658994-95
CGCGGAT35250.07.94844061
Produced by FastQC (version 0.11.5)