FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n02_ALm05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n02_ALm05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35070582
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA61350.014.3339949
GTTACGA32250.012.0792864
TTACGAC35300.010.6336875
CGATCCG46400.010.180478-79
TGTTACG37050.010.127493
TAACACG114200.09.9801022
ACGTGCG139450.09.882716
TCGACCG46050.09.80504926-27
TACGACT41700.09.6867816
GTGCGCG100100.09.4968448
GTGTCGA44000.09.3934194
ATCCGAC46650.09.1682716
AACACGT134600.08.998253
AATCCGA62500.08.9708485
TACGACG47700.08.86914359
CCGGTAT71800.08.8626331
CGGGGTT123800.08.7457411
GTTCGAC55850.08.72169824-25
CGGGAAT69750.08.37421
CGCGTAT8006.483744E-68.3103711
Produced by FastQC (version 0.11.5)