FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n02_ALm04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n02_ALm04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26479546
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA42800.012.4392069
ACGTGCG87900.09.7317926
GTGTCGA33850.08.9822514
CGATCCG34250.08.94042678-79
TCGACCG35550.08.82415426-27
TAACACG79000.08.7761042
AATCCGA44950.08.7739195
GTGCGCG71500.08.7085688
ATCCGAC36850.08.6406526
GCGTCGT146850.08.55044494-95
GTTACGA24650.08.4800744
TTACGAC27500.08.4665915
TGTCGAG39300.08.3426065
GGGGAAT182050.08.2690281
AACACGT87600.08.1870213
GATCGGA369350.08.12298994-95
CGGGAAT48550.08.1184791
CTACCGT31850.08.0587158
GTCGATT18450.07.9790491
CCGGGAT100850.07.86368941
Produced by FastQC (version 0.11.5)