FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n02_ALm03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n02_ALm03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23398871
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA42600.011.3817129
CCGGTAT48050.010.6734131
CGATCCG30200.09.98268478-79
TCGACCG32050.09.41669726-27
CGGTATT51300.09.1642772
ACGTGCG86200.08.8208756
GTGTCGA29300.08.7551484
CGCGTAT4950.0026612988.63396551
GATCGGA316900.08.53894994-95
CGCGGAT26150.08.5349131
TAACACG70900.08.4392662
GTTCGAC35700.08.31961724-25
CCGGGAT88250.08.179031
GTTACGA21050.08.1243334
GTGCGCG66400.08.0175538
CCCGTAT28650.07.9559051
AGATCGG361700.07.907884694-95
TTACGAC23000.07.8501615
TACGACT26250.07.78463276
CTACCGT28750.07.77055268
Produced by FastQC (version 0.11.5)