FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n02_ALm02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n02_ALm02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25810910
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA47350.010.5418389
GTGTCGA32800.09.9940564
TCGACCG34750.09.30118726-27
CGATCCG31700.09.21005278-79
GTTACGA24000.08.9077454
TTACGAC26500.08.7861635
GTCTAAC54550.08.7090694
ATCCGAC32750.08.7068576
GTCGGTA18900.08.54251
CGGGAAT50950.08.481351
CGCGATT10709.0130925E-98.4321371
TGTCGAG40150.08.4027585
GTTCGAC40150.08.34504824-25
TACGACT27750.08.0492686
AATCCGA42100.07.67495545
GTCGAGG59500.07.66788776
CGCGTAT5650.0087479417.56418041
GATCGGA395450.07.55056794-95
TGTTACG27200.07.50832563
TAACACG84900.07.49473862
Produced by FastQC (version 0.11.5)