FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n02_ALm01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n02_ALm01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31148766
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA58000.012.1297559
GTGTCGA39450.010.7177534
GTTACGA30100.09.4699034
ACGTGCG113850.08.8909966
CTACCGT34850.08.8655968
CGGGGTT109650.08.79145051
TTACGAC32400.08.6526145
CCGGGAT122600.08.6374891
TGTTACG32200.08.5552993
TGTCGAG48500.08.5234985
TAACACG100100.08.3968372
CGCCAAT48100.08.3916421
TACGACT36500.08.20266
ATCCGAC41400.08.150086
TCGACCG40000.08.13937426-27
CGGGAAT63950.08.0939231
AACACGT111750.08.0754953
GTGCGCG92500.07.9650288
GCGTCGT310300.07.917066694-95
CGATCCG40050.07.88275878-79
Produced by FastQC (version 0.11.5)