FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35102780
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5400291.5384223129905952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC230300.023.9936188
TCGGAAG256400.021.4655343
CGTCTGA130300.020.60742216-17
ATCGGAA273600.020.4966282
ACGTCTG129750.020.38781214-15
CACGTCT133500.020.27552614-15
CGGAAGA276200.020.2445684
ACACGTC133500.020.1537812-13
GATCGGA264450.020.029021
TACTCGA41100.020.01361836-37
ACTCGAT41250.019.25322736-37
AATCGGG33600.018.36027740-41
CACACGT149450.018.31927112-13
GAATCGG37600.017.22426640-41
CTCGTAT41200.016.86719944-45
GCACACG175450.015.5561510-11
CTGAACT188300.014.46027118-19
CAGTCAC154850.014.27182926-27
ATGCCGT59600.014.19761548-49
TGAACTC194050.014.18978220-21
Produced by FastQC (version 0.11.5)