FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n01_ALm16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n01_ALm16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23056019
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGG90400.029.63368240-41
GAATCGG94600.029.32220840-41
AGAATCG132500.022.49436438-39
CGGAGAA180300.020.29780436-37
ATCGGGG124650.019.60516242-43
CACTCCG191200.018.92913230-31
GTCACTC197800.018.71780428-29
GAGCACA404400.018.4983739
CCGGAGA205950.017.7006534-35
AGAGCAC423350.017.5918528
AATCGCG11350.017.57633840-41
CGTCTGA210950.017.56209816-17
ACTCCGG217050.017.27656632-33
TCCGGAG218950.017.20286834-35
TCACTCC222400.016.94633530-31
ACACGTC216900.016.83945712-13
ACGTCTG218150.016.8191714-15
TCGGAAG437100.016.7133753
CACGTCT227600.016.35038614-15
ATCGGAA453950.015.9678842
Produced by FastQC (version 0.11.5)