FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n01_ALm15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n01_ALm15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26574093
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG100550.028.86347240-41
AATCGGG99400.027.93107240-41
AGAATCG142800.021.8039138-39
CGGAGAA190200.019.7911836-37
GAGCACA401200.018.8711559
TCGGAAG397400.018.6820033
CGTCTGA205800.018.44031716-17
ATCGGAA401850.018.3927252
ACACGTC202050.018.28890612-13
CACTCCG202400.018.11695530-31
AGAGCAC419350.018.0884348
ATCGGGG138100.017.97144142-43
GATCGGA407450.017.9360641
GTCACTC211050.017.91452628-29
ACGTCTG210750.017.59026314-15
CACGTCT216950.017.39407214-15
CGGAAGA433950.017.1633784
CCGGAGA221300.016.93470834-35
TCCGGAG224500.016.7991134-35
AATCGCG13450.016.59835440-41
Produced by FastQC (version 0.11.5)