FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n01_ALm14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n01_ALm14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34241171
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG137800.028.79872740-41
AATCGGG133200.028.67001240-41
AGAATCG187350.022.79199838-39
CGGAGAA249100.020.36434436-37
ATCGGGG176000.019.64704942-43
CACTCCG260700.019.03918830-31
CCGGAGA284250.017.9379834-35
TCCGGAG293300.017.48975234-35
GTCACTC289400.017.3644128-29
ACTCCGG301000.017.18435932-33
AATCGCG17800.017.07796140-41
GAGCACA611500.016.8318779
AGAGCAC642700.015.9186248
TCACTCC329150.015.5632230-31
CGTCTGA333200.015.4878916-17
TCGGAAG655400.015.2633073
CTCCGGA331700.015.25737832-33
ACGTCTG336300.014.98506314-15
ACACGTC338600.014.84818312-13
ATCGGAA670150.014.8212792
Produced by FastQC (version 0.11.5)