FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n01_ALm13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n01_ALm13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31221240
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG108950.027.42246840-41
AATCGGG105050.027.24232540-41
AGAATCG159450.020.21191438-39
CGGAGAA209800.018.6775936-37
TCGGAAG429300.017.902373
ATCGGAA432250.017.582642
GAGCACA449850.017.4216359
GATCGGA433850.017.3708321
ATCGGGG153600.017.13181942-43
CACTCCG219550.017.12335430-31
ACACGTC221750.017.09261512-13
CGTCTGA231900.016.96915816-17
ACGTCTG228700.016.67701114-15
CACGTCT235400.016.6361614-15
AGAGCAC466350.016.6219088
GTCACTC233400.016.43282728-29
CGGAAGA482950.016.0218184
CCGGAGA243000.016.01826334-35
TGCGCGA59050.016.0068519
TCCGGAG251300.015.7916234-35
Produced by FastQC (version 0.11.5)