FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n01_ALm12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n01_ALm12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30409862
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG118550.028.56718440-41
AATCGGG118350.027.2910440-41
AGAATCG166450.021.63043238-39
CGGAGAA221300.020.40075336-37
CACTCCG228000.019.48870730-31
GAGCACA490600.018.830649
GTCACTC248900.018.10028628-29
AGAGCAC510100.017.9524618
ATCGGGG161500.017.82310742-43
TCCGGAG260550.017.76504334-35
CCGGAGA255350.017.70828834-35
CGTCTGA259800.017.66056316-17
TCGGAAG508700.017.582513
ACACGTC255800.017.52828612-13
CACGTCT264850.017.34179914-15
ACGTCTG259200.017.29834714-15
ACTCCGG266250.017.28643432-33
ATCGGAA517850.017.162082
GATCGGA518950.017.0406171
CGGAAGA548500.016.4886364
Produced by FastQC (version 0.11.5)