FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n01_ALm11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n01_ALm11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38301010
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG185100.032.0507640-41
AATCGGG180950.031.36834540-41
AGAATCG261350.024.0355538-39
ATCGGGG228350.023.0785842-43
CGGAGAA347300.021.95062336-37
AATCGCG20650.020.93165840-41
GTCACTC366800.020.31732628-29
GAGCACA766050.019.8284829
CACTCCG372300.019.80671130-31
TCACTCC400000.019.12381430-31
CCGGAGA400150.018.74277734-35
TCCGGAG411450.018.6089834-35
AGAGCAC825400.018.3854858
ACTCCGG430600.018.21145232-33
AGTCACT425500.018.12839728-29
CGTCTGA432900.017.67558516-17
TCGGAAG875150.016.977353
ACGTCTG437950.016.96205114-15
CTCCGGA442350.016.8740732-33
CACGTCT458550.016.73866514-15
Produced by FastQC (version 0.11.5)