FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n01_ALm10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n01_ALm10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19566181
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG77000.029.76436640-41
AATCGGG75800.028.6689640-41
AGAATCG110150.021.94929738-39
CGGAGAA146800.020.4006836-37
CACTCCG152100.019.11190430-31
ATCGGGG105450.019.00887742-43
GAGCACA321050.018.7593759
GTCACTC163650.018.183828-29
AGAGCAC332800.018.0685968
CGTCTGA170700.018.05850816-17
AATCGCG8500.017.60279840-41
TCCGGAG172950.017.59058834-35
TCGGAAG334150.017.5557523
CCGGAGA169050.017.46255134-35
ACACGTC171600.017.29949212-13
ATCGGAA341200.017.1655162
GATCGGA342150.017.0261571
ACTCCGG180150.017.00615332-33
ACGTCTG174600.016.9886514-15
CACGTCT180550.016.98124114-15
Produced by FastQC (version 0.11.5)