FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n01_ALm09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n01_ALm09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25214022
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG110050.031.26991740-41
AATCGGG109700.029.89753540-41
AGAATCG151300.024.439838-39
CGGAGAA201250.021.96105636-37
ATCGGGG143700.020.60909542-43
CACTCCG216850.019.88823130-31
GTCACTC229000.019.19601628-29
CCGGAGA228850.019.15678834-35
TCCGGAG239000.018.78044334-35
AATCGCG13800.018.4142140-41
ACTCCGG249450.018.10784332-33
GAGCACA496400.017.8736469
TCACTCC253550.017.6651730-31
CTCCGGA259100.016.9934732-33
AGAGCAC533700.016.5266048
AGTCACT276050.016.45763428-29
CGTCTGA275150.016.33863316-17
CAGTCAC290950.015.56585426-27
ACGTCTG284150.015.445065514-15
TCGGAAG565100.015.3821823
Produced by FastQC (version 0.11.5)