FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n01_ALm08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n01_ALm08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24563836
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT563910.22956919269449608TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC94000.036.88746346-47
TCGTATG90800.036.8010244-45
CTCGTAT90450.033.92388544-45
TATGCCG102600.033.65695648-49
TACTCGA107100.031.95370736-37
ACTCGAT108250.029.39839636-37
TTACTCG115950.029.10516434-35
ATGCCGT135400.024.83718548-49
TCTCGTA117700.024.7784942-43
GTATGCC138150.024.5488546-47
GCCGTCT144550.023.24891950-51
ATCTCGT129850.022.69774642-43
ATTACTC166200.021.20555334-35
ACATTAC167250.021.1007632-33
TGCCGTC165850.021.03636650-51
TCGATCT141050.020.44078838-39
CTCGATC153350.019.40525238-39
CACATTA181600.019.02797530-31
GAGCACA383600.018.4987939
GATCTCG156400.018.44981640-41
Produced by FastQC (version 0.11.5)