FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n01_ALm07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n01_ALm07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31689468
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT648560.20466105647466218TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG108100.036.55786544-45
CGTATGC113600.036.06337446-47
CTCGTAT110800.033.73790744-45
TATGCCG122150.033.4031248-49
TACTCGA135250.029.83333236-37
ACTCGAT132900.028.2164836-37
TTACTCG144600.027.65782734-35
TCTCGTA145250.024.41163442-43
ATGCCGT167850.023.86992348-49
GTATGCC172450.023.53609846-47
GCCGTCT178200.022.3236150-51
ATCTCGT162450.022.30940642-43
TGCCGTC202150.020.45419150-51
ACATTAC206150.020.33314932-33
ATTACTC206750.020.13634734-35
TCGATCT176550.019.80114238-39
CTCGATC192150.018.8733438-39
CACATTA225850.018.13901330-31
GATCTCG198650.017.77761740-41
CCGTCTT234050.017.42290952-53
Produced by FastQC (version 0.11.5)