FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n01_ALm06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n01_ALm06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32318132
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT804970.24907689590475093TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG128750.037.70403344-45
CGTATGC133750.037.2534546-47
CTCGTAT126800.035.043644-45
TATGCCG144500.034.21913548-49
TACTCGA147550.033.1247236-37
ACTCGAT148600.031.06873336-37
TTACTCG163800.029.91105734-35
ATGCCGT182050.026.63920248-49
GTATGCC189250.025.81387746-47
TCTCGTA167050.025.26355242-43
GCCGTCT190800.025.1563450-51
ATCTCGT180200.023.86806342-43
TGCCGTC216050.023.0297550-51
TCGATCT187700.022.28164338-39
ATTACTC230450.022.14655334-35
ACATTAC232550.022.06892432-33
CTCGATC205400.020.9628138-39
GATCTCG210950.020.1411240-41
CCGTCTT246550.019.93053852-53
CACATTA256750.019.60032530-31
Produced by FastQC (version 0.11.5)