FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n01_ALm05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n01_ALm05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35070582
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT774610.22087172662261495TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG129450.036.4359844-45
CGTATGC133000.036.33847846-47
CTCGTAT125300.034.38266444-45
TATGCCG145600.033.210348-49
TACTCGA149200.032.08968436-37
ACTCGAT147250.030.611536-37
TTACTCG164350.029.11718634-35
ATGCCGT183250.025.92044448-49
GTATGCC193700.024.84069446-47
TCTCGTA168650.024.6858642-43
GCCGTCT197000.024.05108350-51
ATCTCGT184450.022.76440642-43
TGCCGTC222450.021.92936150-51
TCGATCT193400.021.30558838-39
ATTACTC239100.020.77907234-35
ACATTAC243150.020.42311332-33
CTCGATC216500.019.2188238-39
CCGTCTT251000.019.1134652-53
GATCTCG216900.019.10673140-41
CACATTA267450.018.36328730-31
Produced by FastQC (version 0.11.5)