FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n01_ALm04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n01_ALm04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26479546
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT539830.20386678835052535TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG92000.035.65051344-45
CGTATGC95300.035.56232546-47
TATGCCG102700.033.09246448-49
CTCGTAT92150.032.9894144-45
TACTCGA106750.031.30212636-37
ACTCGAT106750.029.29985836-37
TTACTCG120450.027.64317934-35
ATGCCGT135750.024.65080348-49
GTATGCC143450.023.41035346-47
TCTCGTA123700.023.36578442-43
GCCGTCT145500.022.63979750-51
ATCTCGT132350.021.98222442-43
TGCCGTC166500.020.5545950-51
TCGATCT140750.020.18086838-39
ATTACTC176750.019.71140134-35
ACATTAC179700.019.57265332-33
CTCGATC153650.019.1511938-39
GATCTCG161300.017.69815440-41
CCGTCTT192700.017.5628252-53
CACATTA199900.017.11964230-31
Produced by FastQC (version 0.11.5)