FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n01_ALm03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n01_ALm03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23398871
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT544750.23281037790242104TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC91050.035.91757646-47
TCGTATG87900.035.90779544-45
CTCGTAT86450.034.42220744-45
TATGCCG96550.033.94520648-49
TACTCGA102750.031.8503336-37
ACTCGAT103200.029.34114536-37
TTACTCG112300.028.73981334-35
ATGCCGT125750.025.51520248-49
TCTCGTA112350.025.19748542-43
GCCGTCT128300.024.6935350-51
GTATGCC131250.024.59083246-47
ATCTCGT124800.023.02633342-43
TGCCGTC149450.021.94581450-51
TCGATCT135350.020.68743938-39
ATTACTC163200.020.57659334-35
ACATTAC164950.020.34372132-33
CTCGATC149800.019.34190438-39
GATCTCG146150.019.1750440-41
CCGTCTT172900.018.81848552-53
CACATTA181250.018.2390130-31
Produced by FastQC (version 0.11.5)