FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n01_ALm02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n01_ALm02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25810910
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT817680.3167962694844932TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG127600.038.65771544-45
CGTATGC131700.038.2840246-47
CTCGTAT121700.037.2924144-45
TATGCCG140050.035.61174848-49
TACTCGA141650.035.1412436-37
ACTCGAT142750.032.70768436-37
TTACTCG153100.032.2803634-35
ATGCCGT166500.029.55511548-49
TCTCGTA148300.029.19415542-43
GTATGCC173250.028.66382246-47
GCCGTCT175900.027.8136850-51
ATCTCGT160700.027.26659242-43
TGCCGTC193600.025.81056250-51
TCGATCT171650.024.71072638-39
ATTACTC206050.024.67661534-35
ACATTAC209200.024.4867132-33
CTCGATC184850.023.52430238-39
GATCTCG186250.023.07972140-41
GAGCACA457100.022.3512159
CACATTA228000.022.12400430-31
Produced by FastQC (version 0.11.5)