FastQCFastQC Report
Thu 11 Oct 2018
HTW57BGX7_n01_ALm01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW57BGX7_n01_ALm01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31148766
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT894250.28709002468990263TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC145300.038.05105246-47
TCGTATG141900.037.9585544-45
CTCGTAT136300.036.4514544-45
TATGCCG155150.035.4824648-49
TACTCGA168650.032.44456536-37
ACTCGAT165900.030.849436-37
TTACTCG182850.029.84694134-35
GTATGCC195350.027.90092546-47
TCTCGTA171200.027.50858542-43
ATGCCGT197550.027.48218748-49
GCCGTCT205500.026.18805950-51
ATCTCGT186450.025.50063542-43
TGCCGTC229900.023.9871450-51
ATTACTC242350.023.25409734-35
TCGATCT203150.022.95990838-39
ACATTAC247700.022.68465232-33
CTCGATC216550.022.06557738-39
GATCTCG221600.021.30562240-41
CCGTCTT263100.020.91549352-53
CACATTA269100.020.6865330-31
Produced by FastQC (version 0.11.5)