FastQCFastQC Report
Tue 30 Oct 2018
HTW3CBGX7_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW3CBGX7_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357789954
Sequences flagged as poor quality0
Sequence length99
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37684421.0532553968801484No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAACCAACCGGGA5588370.15619136137064374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATG8410850.086.8102658
GCTCGAT8464550.086.242337
GGCTCGA2777500.084.180556
CGCTCGA1835950.081.851276
AGCAGTG13264450.081.471082
AAGCAGT13383600.080.9155961
CGGCTCG621950.078.567415
GGGCTCG1042950.076.199135
CCGCTCG427300.071.748245
AGGCTCG685850.070.6779255
GCAGTGG15601300.069.361423
TCGCTCG595850.068.033835
CAGTGGT16305300.066.3324664
AGCTCGA2278500.065.206566
TGGCTCG894850.064.868455
TCGATGA11386900.064.556259
TGCTCGA3110000.064.471866
GAGCTCG663950.064.283945
AGTGGTA17869000.060.7136045
GTGGTAT19088150.056.8749166
Produced by FastQC (version 0.11.5)