FastQCFastQC Report
Tue 30 Oct 2018
HTW3CBGX7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW3CBGX7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357789954
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGCTC6649197918.58408215676173No Hit
GTCCTTCT6155396817.20393971710005No Hit
AAGACGGA5754246116.082749209889776No Hit
TCTTAAAG4409378912.323931543365804No Hit
AGCGAAAG341906129.556057015508044No Hit
TTTCTGTC269485157.53193729972642No Hit
GAAACCCT259248507.245829490226548No Hit
GGGGGGGG228503886.386537057437895No Hit
TCCGGAGA59519491.6635316149765347No Hit
GGAATCTC19332520.5403315488282268No Hit
CCGTGTGA8553210.23905673997766858No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)