FastQCFastQC Report
Tue 30 Oct 2018
HTW3CBGX7_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW3CBGX7_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357789954
Sequences flagged as poor quality0
Sequence length27
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGG15633930.43695832779027666No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGC6573190.18371644945626395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGC765800.018.69786621
GCGCGAT949200.018.3409421
CAATGGA1384650.018.2251368
CTGCGGA1021250.018.062021
CTCGAAA1119200.017.966771
ACTGATG1072800.017.7119941
GGGAATG902650.017.59851
ATGCGAT974450.017.5121731
GACTGCG1230200.017.4862351
ATAACGC1039550.017.4601611
GATCGCG1150950.017.4127391
GGGCATC1321700.017.3539961
ATGGGAG943850.017.3354951
GGCTCGA1043350.017.2918821
TACGGGC1028450.017.2431871
CTAAGAC985200.017.2240771
GGGACCT699550.017.217441
TGACTAG1019250.017.2040771
CACCGAA701850.017.2021378
CCGGTAG965650.017.1475261
Produced by FastQC (version 0.11.5)