FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23597221
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15011216.361431288879313No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN429220.181894300180517No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN407520.17269830205853479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG169650.013.4361593
CGGAAGA177100.013.2009224
TCGTGTA84650.013.14266814-15
ATCGGAA175800.013.0977452
GATCGGA177350.012.830151
CGTGTAT15850.011.85042414-15
GTAGGGA81150.010.97881618-19
AGCGTCG104850.010.63236910-11
GAGCGTC212350.010.4537559
TAGGGAA81850.010.20829218-19
AGAGCGT231300.09.6988518
CGGGGAT23950.09.6210011
GTGTAGG92750.09.47672216-17
AAGAGCG250650.09.3704637
TGTAGGG90200.09.1038916-17
CCGGGAT26600.07.7601721
CGGGTTT18650.07.721951
GGGAAAT66250.07.6807661
CGGGAAT19500.07.6315311
CGGGCAT33500.07.5947871
Produced by FastQC (version 0.11.5)