FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n02_HBLm14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n02_HBLm14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25508773
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG300250.09.55266680-81
AAGGGGG295550.08.90120982-83
CGGGAAT55350.08.6669941
TAGGGGG114350.08.59604850-51
AAAAGGG302350.08.5518280-81
CCGGGAT75100.08.2218241
AGGGGGG415550.07.987743482-83
GCGTCGT236650.07.979666794-95
AAAAAGG425250.07.922808678-79
CGGGTAT28250.07.73399831
CGTCGTG231600.07.651109794-95
CGCGGAT37300.07.6402421
GATCGGA554400.07.545017794-95
TAAAAAA736650.07.503350370-71
GGCGAAT30600.07.45048481
CCGGAAT58500.07.3883971
ATCGGAA517600.07.356366694-95
AGATCGG617100.07.305749494-95
GCGGGTT42350.07.2899521
CGGGGAT68800.07.2487911
Produced by FastQC (version 0.11.5)