FastQCFastQC Report
Fri 8 Feb 2019
HTW33BGX9_n02_HBLm13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTW33BGX9_n02_HBLm13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25325599
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG273050.08.52159480-81
CGCGAAT8652.0770258E-68.2362431
CGGGGTT88350.08.2250561
CGGGTAT26800.08.1522421
CGCGTAT6504.194621E-48.0377281
CGGAAAT53800.07.9453721
CGATCTA11151.5968544E-77.6696434
GCGTCGT222200.07.64324594-95
CGGGGAT74000.07.63781641
AGGGGGG417050.07.634439582-83
GGGAAAT208150.07.5071221
CTCGGTT49600.07.4690771
AGCGTCG257250.07.44209894-95
GTCGCAT26300.07.40426731
CGACCTA15452.9649527E-107.38006644
CGTCGTG214750.07.344304694-95
AAAAGGG277900.07.32198578-79
ATCGGAA491350.07.299645494-95
CCGGGAT80300.07.2751761
AATCGTG44200.07.09492875
Produced by FastQC (version 0.11.5)